ชุดตรวจ ELISA สำหรับสปีชีส์อื่น
HVD3(25-Hydroxyvitamin D3) ELISA Kit
รหัสสินค้า: RE10127
ผู้ผลิต: Reed Biotech
ราคา: กรุณาสอบถาม
- สปีชีส์
- Universal
- ความไว
- 0.91 ng/mL
- ช่วงการตรวจวัด
- 3.13-200 ng/mL
- ชนิดตัวอย่าง
- Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids,
Product Details
| Product Name | HVD3(25-Hydroxyvitamin D3) ELISA Kit | Species | Universal |
| Uniprot ID | N/A | Alternative Names | 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D |
| Detection method | Competitive | Sensitivity | 0.91 ng/mL |
| Standard | 200 ng/mL | Detection Range | 3.13-200 ng/mL |
| Sample type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL | ||
| Reaction time | 1.5H | Research Area | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
| Test principle | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to HVD3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
| (ng/mL) | OD |
|---|---|
| 200.00 | 0.188 |
| 100.00 | 0.324 |
| 50.00 | 0.539 |
| 25.00 | 0.873 |
| 12.50 | 1.301 |
| 6.25 | 1.693 |
| 3.13 | 2.069 |
| 0.00 | 2.509 |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(ng/mL) | 3.94 | 8.40 | 56.18 | 4.10 | 6.29 | 69.93 |
| Standard deviation | 0.42 | 0.93 | 2.90 | 0.52 | 1.15 | 3.61 |
| C V (%) | 4.32 | 7.49 | 5.36 | 3.39 | 4.44 | 2.32 |
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 80-93 | 85 |
| EDTA plasma (n=8) | 87-99 | 93 |
| Cell culture media (n=8) | 90-103 | 96 |
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 96-101 | 87-98 | 95-102 |
| Average (%) | 98 | 95 | 96 | |
| 1:4 | Range (%) | 85-92 | 87-98 | 89-97 |
| Average (%) | 86 | 97 | 94 | |
| 1:8 | Range (%) | 80-96 | 92-104 | 86-102 |
| Average (%) | 91 | 98 | 99 | |
| 1:16 | Range (%) | 89-101 | 82-96 | 94-106 |
| Average (%) | 99 | 86 | 101 |
Assay Procedures
Citations(1)
IF：8.1
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