DMEM, High Glucose, GlutaPlus, 1.5g/L Sodium Bicarbonate

Product Information

Product Description

Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is now widely used in various cell cultures. DMEM High Glucose Medium is commonly used for fast-growing cells with low adhesion. It can be used for the culture of many kinds of mammalian primary cells, such as primary fibroblasts, neuronal cells, glial cells, HUVEC, smooth muscle cells, etc. It can also be used for the culture of some cell lines, such as Hela, 293, Cos-7 and PC-12.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type. This product is a modified DMEM high glucose medium according to ATCC 30-2002. It contains 1.5 g/L sodium bicarbonate and sodium pyruvate.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 25 mM D-glucose, 1.5 g/L sodium bicarbonate, 4.0 mM L-alanyl-L-glutamine, 1.0 mM sodium pyruvate and phenol red indicator, no HEPES buffer system.

L-alanyl-L-glutamine, a cell culture additive, replaces medium molar L-glutamine in cell culture medium. The substance is very stable in aqueous solution and does not degrade spontaneously, it is slowly degraded by a peptidase secreted by the cells to release glutamine, which is then absorbed and used by the cells.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8℃, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.

3. For your safety and health, please wear safety glasses, gloves or protective clothing.

For Research Use Only!

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