McCoy’s 5A (Modified) Medium, GlutaPlus, HEPES

Product Information

Product Description

McCoy's 5A modified medium is a general-purpose medium originally designed specifically for Novikoff Hepatoma cells by Thomas McCoy et al. McCoy's 5A contains a high concentration of glucose, bacterial peptone and reduced glutathione compared to other media. McCoy’s 5A medium modified generally supports the propagation of many types of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryo and omentum, and other tissues. In addition, McCoy's 5A modified medium is used for tissue biopsy cultures, cell line building, and the culture of some lymphocytes and more difficult cells.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 16.67 mM D-glucose, 1.5 mM L-Alanyl-L-Glutamine, 25 mM HEPES buffer system, phenol red indicator, no sodium pyruvate.

HEPES is a commonly used biological buffer that can keep the culture medium in a constant pH range for a longer period of time, effectively preventing the culture medium from large pH fluctuations that may adversely affect cell growth.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8℃, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

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