การสกัดดีเอ็นเอ (DNA Extraction)
α+ NGS™ Feline Blood Genomic DNA Extraction Mini Kit
รหัสสินค้า: APFBK004 / APFBK050 / APFBK100 / APFBK200
ผู้ผลิต: ALPHAGEN
ขนาด:
4 preps50 preps100 preps200 preps
ราคา: กรุณาสอบถาม
- สปีชีส์
- Cat
- หลักการ
- mini spin column (silica matrix)
- ปริมาณตัวอย่าง
- 1. Up to 200 µl whole blood, serum, plasma, body fluids. 2. Up to 5 x 106 cultured cells.
- เวลาที่ใช้
- 30 ~ 60 minutes
Ordering information
| APFBK004 | 4 preps (sample) |
|---|---|
| APFBK050 | 50 preps |
| APFBK100 | 100 preps |
| APFBK200 | 200 preps |
Storage: Store at room temperature (15~ 25 ℃) for 2 year. Except Proteinase K Powder, store at 4℃.
Component
| APFBK 004 (4 preps) |
APFBK 050 (50 preps) |
APFBK 100 (100 preps) |
APFBK 200 (200 preps) |
|
| FG Buffer | 1.5 ml | 1.5 ml | 30 ml | 60 ml |
| FW Buffer (concentrate) |
1.3 ml | 22 ml | 44 ml | 88 ml |
| F-Wash Buffer (concentrate) |
1.0 ml x 2 | 10 ml x 2 | 20 ml x 2 | 40 ml x 2 |
| Elution Buffer | 1.0 ml | 15 ml | 30 ml | 60 ml |
| Proteinase Kc | 1.0 mg | 11 mg | 11 mg x 2 | 11 mg x 4 |
| FG Mini Column | 4 pcs | 50 pcs | 100 pcs | 200 pcs |
| Collection Tube | 8 pcs | 100 pcs | 200 pcs | 400 pcs |
| Elution Tube | 4 pcs | 50 pcs | 100 pcs | 200 pcs |
| User Manual | 1 | 1 | 1 | 1 |
| Preparation of FW Buffer, F-Wash Buffer and proteinase K solution for first use: | ||||
| CW Buffer (concentrate) | 0.5 ml | 8 ml | 16 ml | 32 ml |
| C-Wash Buffer (concentrate) | 4 ml | 40 ml | 80 ml | 160 ml |
| Proteinase Kc | 0.1 ml | 1.1 ml | 1.1 ml | 1.1 ml |
Brief Prodecure
Specification
Principle: mini spin column (silica matrix)
Sample size: 1. Up to 200 µl whole blood, serum, plasma, body fluids. 2. Up to 5 x 106 cultured cells.
Operation time: 30 ~ 60 minutes
Binding capacity: up to 60 µg total DNA / column
Expected yield: 4 ~ 8 µg / prep
Column applicability: centrifugation and vaccum
Minimum C-Elution volume: 50 µl
Important Notes:
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .
- Add ethanol (96- 100 %) to CW Buffer and C-Wash Buffer when first open.
- Prepare dry baths or water baths before the operation. One to 60℃ for step 2. Lysis.
Documents
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