DNA Clean-Up
α+ Solution™ PCR Clean-Up Mini Kit
Catalog #: APPCK004 / APPCK050 / APPCK200
Manufacturer: ALPHAGEN
Price: on inquiry
- Principle
- spin column (silica matrix)
- Sample size
- up to 100 μl of reaction solution
- Operation time
- 10 ~ 15 min
- Storage
- Store at room temperature (15~ 25 ℃) for 2 year.
Ordering information
| APPCK004 | 4 preps (sample) |
|---|---|
| APPCK050 | 50 preps |
| APPCK200 | 200 preps |
Storage: Store at room temperature (15~ 25 ℃) for 2 year.
Component
| APPCK 004 (4 preps) |
APPCK 050 (50 preps) |
APPCK 200 (200 preps) |
|
| PC Buffer | 1.5 ml x 2 | 30 ml | 110 ml |
| Wash Buffer (concentrate) | 1 ml | 12.5 ml | 45 ml |
| Elution Buffer | 0.5 ml | 5 ml | 20 ml |
| PC Mini Column | 4 pcs | 50 pcs | 200 pcs |
| Collection Tube | 4 pcs | 50 pcs | 200 pcs |
| Elution Tube | 4 pcs | 50 pcs | 200 pcs |
| User Manual | 1 | 1 | 1 |
Brief Prodecure
Specification
※ Principle : spin column (silica matrix)
※ DNA Binding capacity of spin column : 20 μl
※ Sample size : up to 100 μl of reaction solution
※ DNA size : 65 bp ~ 10 kbp
※ Recovery : 90% ~ 95% for PCR clean-up
※ Operation time : 10 ~ 15 min
※ Elution volume : 40 μl
Important Notes :
※ Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffer.
※ Add the required volume of ethanol (96~100%) to Wash Buffer before use.
※ Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.
Documents
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