DNA Extraction
α+ Solution™ Plant Genomic DNA Extraction Mini Kit
Catalog #: APPGK004 / APPGK050 / APPGK100
Manufacturer: ALPHAGEN
Price: on inquiry
- Reactivity
- Plant
- Principle
- mini spin column (silica matrix)
- Sample size
- 1. Wet weight ≦ 100 mg 2. Dry weight ≦ 20 mg
- Operation time
- 30 ~ 60 minutes
Ordering information
| APPGK004 | 4 preps (sample) |
|---|---|
| APPGK050 | 50 preps |
| APPGK100 | 100 preps |
Storage: Store at room temperature (15~ 25 ℃) for 2 year. Except RNase A Powder, store at 4℃.
Component
| APPGK 004 (4 preps) |
APPGK 050 (50 preps) |
APPGK 100 (100 preps) |
|
| PG1 Buffer | 2 ml | 25 ml | 55 ml |
| PG2 Buffer | 1 ml | 8 ml | 15 ml |
| PG3 Buffer (concentrate) | 1.5 ml | 15 ml | 30 ml |
| GW Buffer (concentrate) | 0.8 ml | 13 ml | 26 ml |
| Wash Buffer (concentrate) | 1.5 ml | 15 ml | 30 ml |
| Elution Buffer | 1.5 ml | 15 ml | 30 ml |
| RNase A (lyophilized) | 1.5 mg | 22 mg | 43 mg |
| Filter Column | 4 pcs | 50 pcs | 100 pcs |
| PG Column | 4 pcs | 50 pcs | 100 pcs |
| Collection Tube | 8 pcs | 100 pcs | 200 pcs |
| User Manual | 1 | 1 | 1 |
Brief Prodecure
Specification
※ Principle : mini spin column (silica matrix)
※ Sample size : 1. Wet weight ≦ 100 mg
2. Dry weight ≦ 20 mg
※ Operation time : 30 ~ 60 minutes
※ Binding capacity : up to 60 μg total DNA / column
※ Expected yield : 5 ~ 40 μg /prep
※ Column applicability : centrifugation and vaccum
※ Minimum elution volume : 50 μl
Important Notes :
※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
※ Check PG1 Buffer before use, Warm PG1 Buffer at 60℃ for 5 min if any precipitate formd.
※ Preheat dry baths or water baths to 65℃ before the operation.
※ Add required ethanol (96-100%) to PG3 Buffer, GW and Wash before use.
※ Store RNase A at -20℃ upon recipit of kit. Add sterile ddH2O to RNase A tube to make a 50 mg/ml stock solution. Vortex and make sure that RNase A has been completely dissolved. Store the stock solution at -20℃ .
※ All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.
Documents
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