RNA Extraction
α+ Solution™ Viral Nucleic Acid Extraction Kit
Catalog #: APVNK004 / APVNK050 / APVNK100 / APVNK200
Manufacturer: ALPHAGEN
Price: on inquiry
- Principle
- spin column (silica membrane)
- Sample size
- 150 µl cell-free fluid such as serum, plasma, body fluid and cell cultured supernatant
- Operation time
- 20 min
- Storage
- Store at room temperature (15~ 25 ℃) for 2 year. Except Carrier RNA Powder, store at -20℃
Ordering information
| APVNK004 | 4 preps (sample) |
|---|---|
| APVNK050 | 50 preps |
| APVNK100 | 100 preps |
| APVNK200 | 200 preps |
Storage: Store at room temperature (15~ 25 ℃) for 2 year. Except Carrier RNA Powder, store at -20℃
Component
| APVNK 004 (4 preps) |
APVNK 050 (50 preps) |
APVNK 100 (100 preps) |
APVNK 200 (200 preps) |
|
| VNE Buffer | 1.8 ml x 2 | 35 ml | 70 ml | 140 ml |
| Wash Buffer 1 * (concentrate) | 0.9 ml x 2 | 22 ml | 44 ml | 88 ml |
| Wash Buffer 2 * (concentrate) | 1.5 ml | 20 ml | 40 ml | 40 ml x 2 |
| RNase-free Water | 0.5 ml | 6 ml | 6 ml x 2 | 20 ml |
| Carrier RNA | 0.05 mg | 0.4 mg | 0.8 mg | 1.6 mg |
| VNE Column | 4 pcs | 50 pcs | 100 pcs | 200 pcs |
| Collection Tube | 8 pcs | 100 pcs | 200 pcs | 400 pcs |
| Elution Tube | 4 pcs | 50 pcs | 100 pcs | 200 pcs |
| User Manual | 1 | 1 | 1 | 1 |
Brief Prodecure
Specification
- Principle : spin column (silica membrane)
- Sample size : 150 µl cell-free fluid such as serum, plasma, body fluid and cell cultured supernatant
- Fragment size : 100 bp ~30 kb
- Recovery rate : 80 ~ 90 %
- Binding capacity : 30 ug
- Elution volume : 40 ~ 50 µl
- Operation time : 20 min
Important Notes:
- Make sure everything is RNase-free when handling this system.
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Add 1 ml of VNE Buffer to the tube of lyophilized Carrier RNA, mix well by vortexing and transfer the mixture to the VNE Buffer when first open. Store the Carrier RNA added VNE Buffer at 4 °C.
- Add required ethanol (96-100%) to Wash Buffer 1 and Wash Buffer 2 before use.
- Preheat RNase-free water to 70˚C for elution step.
Trouble shooting
|
Problems |
Possible reasons |
Solutions |
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Low or no yield of genomic DNA |
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Incorrect preparation of Wash Buffer 1 or Wash Buffer 2 |
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Wash Buffer 1 and Wash Buffer 2 is not mixed with ethanol before use |
Make sure that the correct volumes of ethanol (96- 100 %) is added into Wash Buffer 1 and Wash Buffer 2 when first open. Repeat the extraction procedure with a new sample. |
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The volume or the percentage of ethanol is not correct before adding into Wash Buffer 1 and Wash Buffer 2 |
Make sure that the correct volumes of ethanol (96- 100 %) is added into Wash Buffer 1 and Wash Buffer 2 when first use. Repeat the extraction procedure with a new sample. |
|
|
Elution of genomic DNA is not efficient |
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|
RNase-free water not completely absorbed by column membrane |
After RNase-free water is added, stand the VNE Column for 2 min before centrifugation. |
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Column is clogged |
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Sample is too viscous |
Reduce the sample volume. |
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Degradation of eluted DNA |
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Sample is old |
Always use fresh or well-stored sample viral nucleic acid extraction. |
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Documents
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