RPMI-1640 Medium, GlutaPlus, HEPES, Sodium Pyruvate

Product Information

Product Description/Introduction

The RPMI in RPMI-1640 is an acronym for Roswell Park Memorial Institute, which stands for the Roswell Park Memorial Institute, and RPMI-1640 is a cell culture medium developed by the institute. Originally designed specifically for lymphocyte culture, this medium is now widely used for the culture of various normal and cancer cells, especially suspension cells, and is one of the most widely used media.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add 5-10% serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 11.1 mM D-glucose, 2.05 mM L-Alanyl-L-Glutamine, phenol red indicator, HEPES and sodium pyruvate.

L-Alanyl-L-Glutamine is a cell culture additive, replaces the equivalent molar amount of L-glutamine in cell culture medium. It is very stable in aqueous solution and does not degrade spontaneously. Instead, it is slowly degraded to release glutamine through the peptidase secreted by cells, then absorbed and utilized by cells.

HEPES is an excellent biological buffer with no toxic effect on cells, and the medium with HEPES can maintain a constant pH range for a longer period of time.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8℃, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

Videos