Labeling Antibody

β-Tubulin Mouse Monoclonal Antibody(5G3)

Name: β-Tubulin Mouse Monoclonal Antibody(5G3)
Brand: Reed Biotech
SKU: RCA22

Catalog #: RCA22

Manufacturer: Reed Biotech

Size: 50 μL100 μL

Price: on inquiry

Reactivity: Human, Mouse, Rat, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Pig, Insect, Yeast
Applications: WB,IHC,IF
Source: Mouse

Product Details

Product Name	β-Tubulin Mouse Monoclonal Antibody(5G3)	Species	H,M,R,Mk,Dg,C,Hm,Rb,Sh,Pg,Insect,Yeast
Applications	WB,IHC,IF
Condition	1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
Alternative Names	Tubulin beta 3 chain, Tubulin beta III, β III tubulin , βIII tubulin , TUBB4
Store	Store at -20°C. Stable for one year from the date of shipment.
Recommended dilutions	WB 1:5,000 IHC 1:200 IF 1:100-200
Specificity	Antibody can detect endogenous β-tubulin proteins.
Background	Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Molecular Weight	55KD	Isotype	IgG1
GeneID	203068	Protein MW	55KD
SWISS	P07437	Source	Mouse
Antigen	Synthetic Peptide	Category	Monoclonal

Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.
IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
IF analysis of Hela with β-tubulin mouse mAb(Left) and DAPI (Right) diluted at 1:100.

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