Labeling Antibody
PCNA Mouse Monoclonal Antibody(12D10)
Catalog #: RCA23
Manufacturer: Reed Biotech
Size:
50 μL100 μL
Price: on inquiry
- Reactivity
- Human, Mouse, Rat
- Applications
- WB,IHC
- Source
- Mouse
Product Details
| Product Name | PCNA Mouse Monoclonal Antibody(12D10) | Species | H,M,R |
| Applications | WB,IHC | ||
| Condition | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| Alternative Names | Cyclin, proliferating cell nuclear antigen | ||
| Store | Store at -20°C. Stable for one year from the date of shipment. | ||
| Recommended dilutions | WB 1:5,000 IHC 1:200 | ||
| Specificity | Antibody can detect endogenous PCNA proteins. | ||
| Background | PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. | ||
| Molecular Weight | 29KD | Isotype | IgG1 |
| GeneID | 5111 | Protein MW | 29KD |
| SWISS | P12004 | Source | Mouse |
| Antigen | Synthetic Peptide | Category | Monoclonal |
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Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T (4) with PCNA mouse mAb(12D10) diluted at 1:5000. -
IHC staining of Human breast cancer tissue paraffin-embedded with PCNA mouse mAb（12D10）diluted at 1:200
View this product on the manufacturer website (Reed Biotech) ↗
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