Labeling Antibody
β-actin Rabbit Polyclonal Antibody
Catalog #: RCA58
Manufacturer: Reed Biotech
Size:
50 μL100 μL
Price: on inquiry
- Reactivity
- Human, Mouse, Rat, Monkey, Chicken, Hamster, Sheep, Xenopus
- Applications
- WB,IHC,IF
- Source
- Rabbit
Product Details
| Product Name | β-actin Rabbit Polyclonal Antibody | Species | H,M,R,Mk,Ch,Hm,Sh,Xenopus |
| Applications | WB,IHC,IF | ||
| Condition | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| Alternative Names | ACTB, Actin, β actin, 2D4H5, Actin cytoplasmic 1 | ||
| Store | Store at -20°C. Stable for one year from the date of shipment. | ||
| Recommended dilutions | WB 1:3,000 IHC 1:200 IF 1:200 | ||
| Specificity | Antibody can detect endogenous β-actin proteins. | ||
| Background | β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues. | ||
| Molecular Weight | 43KD | Isotype | IgG |
| GeneID | 60 | Protein MW | 43KD |
| SWISS | P60709 | Source | Rabbit |
| Antigen | Recombinant Protein | Category | Polyclonal |
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Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000. -
IHC staining of Human ovary tissue with β-Actin pAb diluted at 1:200.
View this product on the manufacturer website (Reed Biotech) ↗
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