
Cell Function Reagents
Ultra Cell Counting Kit-8(CCK-8)Assay Kit
Catalog #: RCK001
Manufacturer: Reed Biotech
Size:
10000 T1000 T
Price: on inquiry
- Sample Type
- Cell
- Applications
- Cell Multiplication
- Method
- Colorimetric method
Product Details
| Product Name | Ultra Cell Counting Kit-8(CCK-8)Assay Kit | Applications | Cell Multiplication |
| Detection principle | In the presence of the electron mediator 1-Methoxy PMS, WST-8 can be reduced by certain mitochondrial dehydrogenases to produce a water-soluble orange-yellow formazan (see Figure 1). The intensity of the formazan color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. The optical density (OD) is measured at 450 nm using a microplate reader, which indirectly reflects the number of living cells. Compared to the basic CCK-8 kit, this ultrasensitive version offers higher sensitivity, significantly stronger signal, and rapid reaction (most cells show noticeable color change after approximately 30 minutes of incubation). It also has a broader linear range, effectively improving experimental efficiency. It is suitable for assays of cell proliferation, cytotoxicity, drug screening, and inhibition of cell growth. | ||
| Detection method | Colorimetric method | Sample type | Cell |
| Detection time | 0.5-2 Hours | Testing instrument | Microplate Reader |
| fluorescence | N/A | Ex/Em (nm) | 450nm |
| Channel set | N/A | Self-supplied reagent | N/A |
| Storage temperature | 2~8°C, shading light | Transport condition | Blue ice |
| Shelf life | 12 months | ||
Citations(2)
AI-Driven Multiscale Study on the Mechanism of Polygonati Rhizoma in Regulating Immune Function in STAD
IF:4.0
Journal:ACS Omega
Species:Human
Sample Types:HGC-27 Gastric Cancer Cells
Novel Ce/Cu-modified black ceramics disrupted mitochondrial dysfunction and tricarboxylic acid cycle to induce cuproptosis in osteosarcoma cells
IF:6.0
Journal:Biomaterials Advances
Species:
Sample Types:
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