การสกัดดีเอ็นเอ (DNA Extraction)
α+ Solution™ Blood / Cultured Cell Genomic DNA Extraction Mini Kit
รหัสสินค้า: APBGR004 / APBGR100 / APBGR200
ผู้ผลิต: ALPHAGEN
ราคา: กรุณาสอบถาม
- หลักการ
- mini spin column (silica matrix)
- ปริมาณตัวอย่าง
- 1. Up to 300 μl of Whole blood 2. Up to 200 μl of frozen blood 3. Up to 200 μl of buffy coat 4. Up to 1 x 10^7 of Cultured animal cells 5. …
- เวลาที่ใช้
- 30 minutes
- การเก็บรักษา
- Store at room temperature (15~ 25 ℃) for 2 year.
Ordering information
| APBGR004 | 4 preps (sample) |
|---|---|
| APBGR100 | 100 preps |
| APBGR200 | 200 preps |
Storage: Store at room temperature (15~ 25 ℃) for 2 year.
Component
| APBGR 004 (4 preps) |
APBGR 100 (100 preps) |
APBGR 200 (200 preps) |
|
| RBC Lysis Buffer | 7 ml | 135 ml | 270 ml |
| TG Buffer | 1.5 ml | 30 ml | 60 ml |
| BG Buffer | 1.5 ml | 40 ml | 80 ml |
| BW Buffer | 1.5 ml x 2 | 45 ml | 90 ml |
| Wash Buffer (concentrate) | 1 ml | 25 ml | 35 ml |
| Elution Buffer | 1.5 ml | 30 ml | 60 ml |
| BG Mini Column | 4 pcs | 100 pcs | 200 pcs |
| Collection Tube | 8 pcs | 200 pcs | 400 pcs |
| User Manual | 1 | 1 | 1 |
Brief Prodecure
Specification
※ Principle : mini spin column (silica matrix)
※ Sample size : 1. Up to 300 μl of Whole blood
2. Up to 200 μl of frozen blood
3. Up to 200 μl of buffy coat
4. Up to 1 x 107 of Cultured animal cells
5. Up to 1 x 109 of Cultured bacterial cells
※ Operation time : 30 minutes
※ Binding capacity : up to 60 μg total DNA / column
※ Expected yield : 4 ~ 8 μg / prep
※ Column applicability : centrifugation and vaccum
※ Minimum elution volume : 50 μl
Important Notes :
※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
※ Add ethanol (96- 100 %) to Wash Buffer when first open.
Trouble shooting
|
Problems |
Possible reasons |
Solutions |
|
Low or no yield of genomic DNA |
||
|
Incorrect preparation of Wash Buffer. |
Wash Buffer is not mixed with ethanol before use. |
Make sure that the correct volumes of ethanol (96 - 100 %) is added into the Wash Buffer when first opened. |
|
The volume or the percentage of ethanol is not correct before adding it into the Wash Buffer. |
Make sure that the correct volumes of ethanol (96 - 100 %) is added into the Wash Buffer when first used. |
|
|
Elution of genomic DNA is not efficient. |
The Elution Buffer is not completely absorbed by the column membrane. |
After the Elution Buffer is added, stand the BG Column for 2 min before centrifugation. |
|
The pH value of Elution Buffer is not correct. |
Make sure the pH value of Elution Buffer is between 7.5- 8.5. |
|
|
Column is clogged |
||
|
Sample is too viscous. |
Reduce the sample volume. |
|
|
Degradation of eluted DNA |
||
|
Sample is old. |
Always use fresh or well-stored sample viral nucleic acid extraction. |
|
Documents
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