การสกัดอาร์เอ็นเอ (RNA Extraction)
α+ Solution™ Plant Total RNA Mini Kit
รหัสสินค้า: APPRK004 / APPR050 / APPRK100
ผู้ผลิต: ALPHAGEN
ราคา: กรุณาสอบถาม
- สปีชีส์
- Plant
- หลักการ
- mini spin column (silica matrix)
- ปริมาณตัวอย่าง
- up to 100 mg plant tissue or 1 x10^7 plant cells
- เวลาที่ใช้
- 30 ~ 60 minutes
Ordering information
| APPRK004 | 4 preps (sample) |
|---|---|
| APPR050 | 50 preps |
| APPRK100 | 100 preps |
Storage: Store at room temperature (15~ 25 ℃) for 2 year.
Component
| APPRK 004 (4 preps) |
APPRK 050 (50 preps) |
APPRK 100 (100 preps) |
|
| RB Buffer | 1.5 ml x 2 | 30 ml | 60 ml |
| PRB Buffer | 1.5 ml x 2 | 30 ml | 60 ml |
| Wash Buffer 1 | 1.5 ml x 2 | 30 ml | 60 ml |
| Wash Buffer 1 * (concentrate) | 1.5 ml | 30 ml | 60 ml |
| Wash Buffer 2 * (concentrate) | 1.5 ml | 20 ml | 35 ml |
| RNase-free Water | 0.5 ml | 6 ml | 6 ml |
| Filter Column | 4 pcs | 50 pcs | 100 pcs |
| RB Mini Column | 4 pcs | 50 pcs | 100 pcs |
| Collection Tube | 8 pcs | 100 pcs | 200 pcs |
| Elution Tube | 4 pcs | 50 pcs | 100 pcs |
| User Manual | 1 | 1 | 1 |
Brief Prodecure
Specification
※ Principle : mini spin column (silica matrix)
※ Sample size : up to 100 mg plant tissue or 1 x107 plant cells
※ Operation time : 30 ~ 60 minutes
※ Binding capacity : up to 100 μg total RNA / column
※ Expected yield : 5 ~ 30 μg of total RNA from 100 mg of young leave
※ Column applicability : centrifugation and vaccum
※ Minimum elution volume : 30 μl
Important Notes :
※ Make sure everything is RNase-free when handling RNA.
※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
※ Caution: ß-mercaptoethanol is hazardous to human health. Perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood.
※ Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
※ Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.
Trouble shooting
|
Problems |
Possible reasons |
Solutions |
|
Low or no yield of RNA |
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|
Incorrect preparation of Wash Buffer 2. |
||
|
Wash Buffer 2 is not mixed with ethanol before use. |
Make sure that the correct volumes of ethanol (96- 100 %) is added into Wash Buffer 2 when first opened. |
|
|
The volume or the percentage of ethanol is not correct before adding into Wash Buffer 2. |
Make sure that the correct volumes of ethanol (96- 100 %) is added into Wash Buffer 2 when first used. |
|
|
Elution of genomic RNA is not efficient. |
||
|
RNase-free water is not completely absorbed by column membranes. |
After RNase-free water is added, stand the RB Column for 1 min before centrifugation. |
|
|
Column is clogged |
||
|
Sample is too viscous. |
Reduce the sample volume. |
|
|
Degradation of eluted RNA |
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|
Sample is old. |
Always use fresh samples. |
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