การสกัดดีเอ็นเอ (DNA Extraction)
α+ Solution™ Tissue Genomic DNA Extraction Mini Kit
รหัสสินค้า: APTGK004 / APTGK050 / APTGK100 / APTGK200
ผู้ผลิต: ALPHAGEN
ราคา: กรุณาสอบถาม
- หลักการ
- mini spin column (silica matrix)
- ปริมาณตัวอย่าง
- 1. Up to 25 mg animal tissue 2. 1.2 cm mouse tail 3. Up to 10^7 cultured cells
- เวลาที่ใช้
- 30 ~ 60 minutes
- การเก็บรักษา
- Store at room temperature (15~ 25 ℃) for 2 year. Except Proteinase K Powder, store at 4℃.
Ordering information
| APTGK004 | 4 preps (sample) |
|---|---|
| APTGK050 | 50 preps |
| APTGK100 | 100 preps |
| APTGK200 | 200 preps |
Storage: Store at room temperature (15~ 25 ℃) for 2 year. Except Proteinase K Powder, store at 4℃.
Component
| APTGK 004 (4 preps) |
APTGK 050 (50 preps) |
APTGK 100 (100 preps) |
APTGK 200 (200 preps) |
|
| TG1 Buffer | 1.5 ml | 15 ml | 30 ml | 60 ml |
| TG2 Buffer | 1.5 ml | 15 ml | 30 ml | 60 ml |
| Proteinase K (lyophilized) |
1 mg | 11 mg | 11 mg x 2 | 11 mg x 4 |
| GW Buffer (concentrate) |
1.3 ml | 22 ml | 44 ml | 88 ml |
| Wash Buffer (concentrate) |
1 ml | 10 ml | 20 ml | 40 ml |
| Elution Buffer | 1 ml | 15 ml | 30 ml | 60 ml |
| TG Mini Column | 4 pcs | 50 pcs | 100 pcs | 200 pcs |
| Collection Tube | 8 pcs | 100 pcs | 200 pcs | 400 pcs |
| Elution Tube | 4 pcs | 50 pcs | 100 pcs | 200 pcs |
| User Manual | 1 | 1 | 1 | 1 |
Brief Prodecure
Specification
※ Principle : mini spin column (silica matrix)
※ Sample size : 1. Up to 25 mg animal tissue
2. 1.2 cm mouse tail
3. Up to 107 cultured cells
※ Operation time : 30 ~ 60 minutes
※ Binding capacity : up to 60 µg total DNA / column
※ Expected yield : 15 ~35 μg / prep
※ Column applicability : centrifugation and vaccum
※ Minimum elution volume : 50 μl
Important Notes :
※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
※ Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .
※ Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
※ Prepare dry baths or water baths before the operation. One to 60℃ for step 2.1 and the other to 70℃ for step 2.2.
※ Preheat the Elution Buffer to 70℃ for step 7.
※ All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.
Documents
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